Investigating the action of the microalgal pigment marennine on Vibrio splendidus by in vivo 2H and 31P solid-state NMR

Bouhlel, Zeineb, Arnold, Alexandre A., Deschênes, Jean-Sébastien, Mouget, Jean-Luc, Warschawski, Dror E., Tremblay, Réjean ORCID: https://orcid.org/0000-0003-2590-8915 et Marcotte, Isabelle (2021). Investigating the action of the microalgal pigment marennine on Vibrio splendidus by in vivo 2H and 31P solid-state NMR. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1863 (9). p. 183642.

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Résumé

This work investigates the potential probiotic effect of marennine - a natural pigment produced by the diatom Haslea ostrearia - on Vibrio splendidus. These marine bacteria are often considered a threat for aquaculture; therefore, chemical antibiotics can be required to reduce bacterial outbreaks. In vivo 2H solid-state NMR was used to probe the effects of marennine on the bacterial membrane in the exponential and stationary phases. Comparisons were made with polymyxin B (PxB) - an antibiotic used in aquaculture and known to interact with Gram(−) bacteria membranes. We also investigated the effect of marennine using 31P solid-state NMR on model membranes. Our results show that marennine has little effect on phospholipid headgroups dynamics, but reduces the acyl chain fluidity. Our data suggest that the two antimicrobial agents perturb V. splendidus membranes through different mechanisms. While PxB would alter the bacterial outer and inner membranes, marennine would act through a membrane stiffening mechanism, without affecting the bilayer integrity. Our study proposes this microalgal pigment, which is harmless for humans, as a potential treatment against vibriosis. -- Keywords : Antimicrobial pigment ; Polymyxin B ; Membrane fluidity ; Model membranes ; 31P and 2H NMR ; In-cell NMR.

Type de document :	Article
Validation par les pairs :	Oui
Information complémentaire :	Voici la version suivant la révision par les pairs, mais précédant la mise en page officielle d’un article publié dans Biochimica et Biophysica Acta (BBA)- Biomembranes. La version finale est disponible en ligne : https://doi.org/10.1016/j.bbamem.2021.183642 This is a post-peer-review, pre-copyedit version of an article published in Biochimica et Biophysica Acta (BBA)- Biomembranes. The final version is available online at: https://doi.org/10.1016/j.bbamem.2021.183642
Mots-clés :	Marennine ; Pigments ; Antibactériens ; Effet ; Bactéries ; Vibrio splendidus ; Membrane cellulaire ; Résonance magnétique nucléaire.
Départements et unités départementales :	Institut des sciences de la mer de Rimouski (ISMER)
Date de dépôt :	12 sept. 2022 19:02
Dernière modification :	05 oct. 2023 15:05
URI :	https://semaphore.uqar.ca/id/eprint/2067