Scalable purification of marennine and other exopolymers from diatom Haslea ostrearia's “blue water”

Bélanger, William, Saint-Louis, Richard, Genard, Bertrand, Deschênes, Jean-Sébastien et Tremblay, Réjean ORCID: https://orcid.org/0000-0003-2590-8915 (2025). Scalable purification of marennine and other exopolymers from diatom Haslea ostrearia's “blue water”. Algal Research, 86 .

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Résumé

Marennine is a bioactive, polydisperse and polyanionic compound produced by certain benthic diatoms of the Haslea genus. Notably, Haslea ostrearia are cultivated in photobioreactors, and their supernatant is collected to extract extracellular marennine (EMn). EMn, a promising blue pigment, has several potential applications, including serving as a pathogenicity-reducing agent in the seafood industry. Nonetheless, its extraction and characterization remain challenging. To meet the need for an efficient, standardized and scalable preparative method, we introduce an improved route to concentrate and purify marennine, prioritizing cost-efficiency and process innocuity. As an exhaustive overhaul of our preliminary study for solid-phase extraction of EMn on synthetic graphitized carbon black, we revised every step to minimize solvent and reagents inputs, while enhancing the purity of the final products. Our improved solid-phase extraction (SPE) method employs natural graphite flakes as the stationary phase, from which the crude hydroorganic extract undergoes purification through ionotropic gelation, followed by chelator-assisted dissolution and cascade diafiltration. Extraction conditions are mild and maintain the ionization state of marennine throughout the process, also allowing for the reuse of both the stationary and the mobile phases. Upon optimization, the yield of the crude extract has been shown to reach up to 87 (6) %. The versatility of the novel process is further evidenced by the isolation of four distinct marennine groups, each with a different molecular weight, as well as four additional extracellular polymeric substances from the culture supernatant. Among these, the EMn fraction with a molecular weight range of 3–1 kDa achieved a mass absorptivity of 13.4 (0.6) L·cm−1·g−1, marking the highest level of purity reported thus far.

Type de document :	Article
Validation par les pairs :	Oui
Mots-clés :	Exopolymères ; Extraction en phase solide ; Graphite ; Gélification ionotropique ; Diatomée / Exopolymers ; Solid-phase extraction ; Graphite ; Ionotropic gelation ; Diatom.
Départements et unités départementales :	Institut des sciences de la mer de Rimouski (ISMER)
Date de dépôt :	13 mai 2025 18:35
Dernière modification :	13 mai 2025 18:35
URI :	https://semaphore.uqar.ca/id/eprint/3306