Evaluation of different extractions for the metabolite identification of malachite green in brook trout and shrimp

Baesu, Anca, Audet, Céline ORCID: https://orcid.org/0000-0003-3366-210X et Bayen, Stéphane (2022). Evaluation of different extractions for the metabolite identification of malachite green in brook trout and shrimp. Food Chemistry, 369 (130567).

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Résumé

Applications of mass spectrometry-based metabolomics in food science have developed fast in the last decade. Sample preparation and data processing are critical in non-target/metabolomic workflows but there is currently no standardized protocol for the development of these methods. The impact of data processing parameters or the inclusion of a different matrix is not often taken into account during the selection of an extraction. Thus, this study aimed to investigate the impact of different extractions, e.g., QuEChERS, and data processing on the determination of malachite green metabolites in two different organisms, brook trout and shrimp. The results obtained confirm the need for a harmonized approach for the validation of non-target workflows, as depending on the comparison criteria, the matrix, the mode of ionization or data processing, a different extraction could be chosen. This study also identified for the first time des-methylated leucomalachite green as another metabolite in the two organisms. -- Keywords : Metabolites ; Aquaculture ; Veterinary drugs ; Anti-fungal treatment ; Extraction ; Suspect screening.

Type de document :	Article
Validation par les pairs :	Oui
Information complémentaire :	CC BY-NC-ND
Départements et unités départementales :	Institut des sciences de la mer de Rimouski (ISMER)
Date de dépôt :	22 juin 2023 12:25
Dernière modification :	05 oct. 2023 15:38
URI :	https://semaphore.uqar.ca/id/eprint/2349