Effects of algae-enriched rotifers on winter flounder (Pseudopleuronectes americanus) gene expression during metamorphosis

Vagner, Marie, de Montgolfier, Benjamin, Sévigny, Jean-Marie, Tremblay, Réjean ORCID: https://orcid.org/0000-0003-2590-8915 et Audet, Céline ORCID: https://orcid.org/0000-0003-3366-210X (2014). Effects of algae-enriched rotifers on winter flounder (Pseudopleuronectes americanus) gene expression during metamorphosis. Marine Biology, 161 (5). pp. 985-999.

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Résumé

The aim of this study was to evaluate the effect of a dietary highly unsaturated fatty acid (HUFA) deficiency on winter flounder Pseudopleuronectes americanus metamorphosis by examining the growth and the expression of genes involved in some key metabolic processes: lipid digestion, oxidative stress, and growth. Three groups of fish were fed rotifers enriched with different blends of microalgae providing different HUFA profiles: (1) a diet comprising a mixture of three microalgae, Nannochloropsis oculata, Isochrysis galbana, and Pavlova lutheri (Cocktail diet), which contained a balanced combination of ecosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and arachidonic acid (AA); (2) the N. oculata diet (Nanno diet), with a low level of DHA; and (3) the I. galbana diet (Tiso diet), characterized by low levels of EPA and AA. The results indicate that the need for DHA increased from settlement and for EPA and AA from 15 days after settlement. The lower HUFA content in the Tiso and Nanno diets did not affect larval development or lipid reserve accumulation. The superoxide dismutase gene expression suggests a reduced oxidative stress in the Cocktail group, and overall results indicate that gh gene expression could be a valuable indicator of development at the molecular level in response to dietary HUFA quality during metamorphosis in winter flounder. -- Keywords : Essential Fatty Acid ; Highly Unsaturated Fatty Acid ; Pelagic Larva ; Lipid Digestion ; Nannochloropsis Oculata.

Type de document :	Article
Validation par les pairs :	Oui
Mots-clés :	Plie Rouge Pseudopleuronectes Americanus Effet Alimentation Diete Acide Gras Insature Developpement Metamorphose
Version du document déposé :	Post-print (version corrigée et acceptée)
Départements et unités départementales :	Institut des sciences de la mer de Rimouski (ISMER)
Déposé par :	DIUQAR UQAR
Date de dépôt :	25 févr. 2019 14:57
Dernière modification :	05 oct. 2023 15:56
URI :	https://semaphore.uqar.ca/id/eprint/1435

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