Influence of generations in captivity, life stage and temperature on gene expression in brain tissue of juvenile brook charr (Salvelinus fontinalis)

Banousse, Ghizlane ORCID: https://orcid.org/0009-0004-9769-5343, Audet, Céline ORCID: https://orcid.org/0000-0003-3366-210X, Lagneau, Angélina, Wilder, Alex, Wilson, Chris C. et Semeniuk, Christina A.D. (2026). Influence of generations in captivity, life stage and temperature on gene expression in brain tissue of juvenile brook charr (Salvelinus fontinalis). Aquaculture, 623 (744124).

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Résumé

Extended captivity in the stable, low-stress conditions of hatcheries can result in adaptive responses that reduce the phenotypic plasticity of fish species, limiting their ability to respond to environmental changes. This may negatively impact the survival, growth, and behavior of fry when released into natural habitats. Here, we examined differences in the neural transcription of 18 genes involved in four key biological processes (stress response regulation, cellular stress response, appetite regulation, and neurogenesis) in fry and fingerling of brook charr (Salvelinus fontinalis) reared at two temperatures (7 °C and 10 °C) and originating from two captive strains of the same genetic origin which differ by the number of generations spent in captivity: the Hill's Lake strain, a long-established captive strain (F > 25), and a recently-established (F1) strain from Scott Lake in Algonquin Park, ON, with substantial Hill's Lake ancestry. The objective was to evaluate whether the transcriptional plasticity of brook charr in response to rearing temperature differences is influenced by domestication across different life stages and to determine whether this plasticity is driven by environmental, genetic, or parental factors. Our findings revealed that multigenerational domestication reduces the threshold for initiating stress response, as evidenced by the upregulation of cellular stress response-related genes in the most domesticated strain. Both groups exhibited similarly low heritability estimates for gene expression variation, indicating a limited long-term adaptation potential under elevated temperature conditions.

Type de document :	Article
Validation par les pairs :	Oui
Mots-clés :	Domestication ; Expression génique ; Température ; Omble de fontaine ; Héritabilité / Domestication ; Gene expression ; Temperature ; Brook charr ; Heritability .
Départements et unités départementales :	Institut des sciences de la mer de Rimouski (ISMER)
Date de dépôt :	28 sept. 2026 17:45
Dernière modification :	29 sept. 2026 19:17
URI :	https://semaphore.uqar.ca/id/eprint/3651